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【英文标准名称】:StandardTestMethodforDeterminingtheAntimicrobialActivityofImmobilizedAntimicrobialAgentsUnderDynamicContactConditions
【原文标准名称】:测定固定抗菌剂在动态接触条件下的抗菌活性的标准试验方法
【标准号】:ASTME2149-2010
【标准状态】:现行
【国别】:美国
【发布日期】:2010
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:E35.15
【标准类型】:(TestMethod)
【标准水平】:()
【中文主题词】:
【英文主题词】:antimicrobialactivity;Disinfectant;bacterial;treatedfiber;fabric
【摘要】:Theantimicrobialactivityofasubstrate-boundantimicrobialisdependentupondirectcontactofmicrobeswiththeactivechemicalagent.Thistestdeterminestheantimicrobialactivityoftreatedspecimenbyshakingsamplesofsurfaceboundmaterialsinaconcentratedbacterialsuspensionforaonehourcontacttimeorothercontacttimesasspecifiedbytheinvestigator.Thesuspensionisseriallydilutedboth.beforeandaftercontactandcultured.Thenumberofviableorganismsinthesuspensionisdeterminedandthepercentreductioniscalculatedbasedoninitialcountsoronretrievalsfromappropriateuntreatedcontrols.Note28212;Thismethodisintendedforthosesurfaceshavingapercentreductionactivityof50%to100%forthespecifiedcontacttime.1.1Thistestmethodisdesignedtoevaluatetheresistanceofnon-leachingantimicrobialtreatedspecimenstothegrowthofmicrobesunderdynamiccontactconditions.Thisdynamicshakeflasktestwasdevelopedforroutinequalitycontrolandscreeningtestsinordertoovercomedifficultiesinusingclassicalantimicrobialtestmethodstoevaluatesubstrate-boundantimicrobials.Thesedifficultiesincludeensuringcontactofinoculumtotreatedsurface(asinAATCC100),flexibilityofretrievalatdifferentcontacttimes,useofinappropriatelyappliedstaticconditions(asinAATCC147),sensitivity,andreproducibility.Thistestalsoallowsfortheversatilityoftestingcontaminationduetosuchthingsashardwater,proteins,blood,serum,variouschemicals,andothercontaminatesorphysical/chemicalstressesormanipulationsofthespecimensofinterest.1.2Surfaceantimicrobialactivityisdeterminedbycomparingresultsfromthetestsampletosimultaneouslyruncontrols.1.3Thepresenceofaleachingantimicrobialisbothpre-andpost-determinedbythepresenceofazoneofinhibition.1.4Thistestmethodshouldbeperformedonlybythosetrainedinmicrobiologicaltechniques.1.5Thisstandardmayinvolvehazardousmaterials,operations,andequipment.Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.
【中国标准分类号】:C10
【国际标准分类号】:19_040
【页数】:4P.;A4
【正文语种】:英语